A Root Structure Architecture (Rsa) Study On Eggplant (Solanum melongena L.) Inoculated With Arbuscular Mycorrhizal Fungi  (AMF) Biofertilizer

Mikee Louissa  Yanez; Diana P Paguntalan

doi:10.58222/jtm.v2i2.51

Authors

Mikee Louissa Yanez Division of Biological Sciences, College of Arts and Sciences University of the Philippines Visayas, Miagao, Iloilo, Philippines
Diana P Paguntalan Division of Biological Sciences, College of Arts and Sciences University of the Philippines Visayas, Miagao, Iloilo, Philippines

DOI:

https://doi.org/10.58222/jtm.v2i2.51

Keywords:

Solanum melongena, arbuscular mycorrhizal fungi, root structure architecture, root colonization, biofertilizer

Abstract

Solanum melongena L. is among the most economically valued horticultural crops globally. In the Philippines, the annual production of this staple crop increases continually. However, several biotic and abiotic factors deteriorate its overall growth and productivity. Recently, the use of sustainable biofertilizers such as arbuscular mycorrhizal fungi (AMF) is gaining interest because of their beneficial impacts on overall plant productivity. One way of examining plant productivity is through root structure architecture (RSA) assessment. Hence, this study aimed to investigate the effects of AMF on the RSA of eggplants to supplement valuable data on its beneficial effects as a biofertilizer. Seedlings were inoculated with four treatment conditions AMF + vermicompost (AMF + V), AMF alone (AMF), NPK fertilizer (NPK), and native soil (C) followed by RSA assessment Results showed that treatments AMF + V and AMF alone significantly promoted lateral root branching. Furthermore, the total diameter and surface area showed a significant increase under treatment AMF + V. Overall, the mycorrhizal-root association presented enhancement towards eggplant RSA which strongly establishes the efficacy of AMF as a promising solution in promoting sustainable agriculture.

References

Aguilar, E., Elleva, L. I. F., Garcia, G. R., Fabro, D. M. A., Divina, F. A., II, & Aggangan, N. (2018). Arbuscular mycorrhizal fungi increased root-mycorrhizal association and enhanced seedling growth of abaca, papaya, and sugarcane. Journal of the International Society for Southeast Asian Agricultural Sciences, 24(2), 22–29. http://issaasphil.org/wp-content/uploads/2019/01/3.-AGUILAR-et-al-2018-Seedling-Inoculam-Mycovam-FINAL.pdf

Alabdallah, N. M., & Alluqmani, S. M. (2022). The synthesis of polysaccharide crude nanoparticles extracts from Taif rose petals and its effect on eggplant seedlings under drought and salt stress. Journal of King Saud University - Science, 34(5), 102055. https://doi.org/10.1016/j.jksus.2022.102055

Bernaola, L., Cosme, M., Schneider, R. W., & Stout, M. (2018). Belowground inoculation with arbuscular mycorrhizal fungi increases the local and systemic susceptibility of rice plants to different pest organisms. Frontiers in Plant Science, 9. https://doi.org/10.3389/fpls.2018.00747

Bhatti, K. H., Kausar, N., Rashid, U., Hussain, K., Nawaz, K., & Siddiqi, E. H. (2013). Effects of biotic stresses on egg plant (Solanum melongena L.). World Applied Sciences Journal, 26(3), 302–311. https://doi.org/10.5829/idosi.wasj.2013.26.03.13460

Böhm, W. (1979). Root parameters and their measurement. Methods of Studying Root Systems, 125–138. https://doi.org/10.1007/978-3-642-67282-8_12

Caruso, T., Mafrica, R., Bruno, M., Vescio, R., & Sorgonà, A. (2021). Root architectural traits of rooted cuttings of two fig cultivars: Treatments with arbuscular mycorrhizal fungi formulation. Scientia Horticulturae, 283, 110083. https://doi.org/10.1016/j.scienta.2021.110083

Chupungco, A. R., Elazegui, D. D., & Nguyen, M. R. (2011). Seed system, production, and marketing of eggplant in three major producing provinces in the Philippines. Philippine Journal of Crop Science, 36(2), 37–47. https://www.cabi.org/gara/mobile/FullTextPDF/2011/20113312474.pdf

De Bauw, P., Ramarolahy, J. A., Senthilkumar, K., Rakotoson, T., Merckx, R., Smolders, E., Van Houtvinck, R., & Vandamme, E. (2020). Phenotyping root architecture of soil-gown rice: A robust protocol combining manual practices with image-based analyses. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.05.13.088369

Elleva, L. I. F., Garcia, G. R., Divina, F. A., II, Fabro, D. M. A., Aguilar, E. A., & Agangan, N. S. (2018). Response of MYKOVAM treated Lakatan banana plantlets to water deficit. Philippine Journal of Crop Science, 43(2), 56–62. https://www.cabi.org/gara/FullTextPDF/2018/20183300826.pdf

Ghani, M. F. A., Ali, A. T., Atif, M., Ali, M., Amin, B., & Cheng, Z. (2022). arbuscular mycorrhizal fungi and dry raw garlic stalk amendment alleviate continuous monocropping growth and photosynthetic declines in eggplant by bolstering its antioxidant system and accumulation of osmolytes and secondary metabolites. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.849521

Goss, M. J., Carvalho, M. A. P. V., & Brito, I. M. (2017). Impacts on host plants of interactions between AMF and other soil organisms in the rhizosphere. Functional Diversity of Mycorrhiza and Sustainable Agriculture. https://doi.org/10.1016/b978-0-12-804244-1.00005-8

Hashem, A., Abd_Allah, E. F., Alqarawi, A. A., Al-Huqail, A. A., & Shah, M. A. (2016). Induction of osmoregulation and modulation of salt stress in Acacia gerrardii Benth. by arbuscular mycorrhizal fungi and Bacillus subtilis (BERA 71). BioMed Research International, 2016, 1–11. https://doi.org/10.1155/2016/6294098

International Service for the Acquisition of Agri-biotech Applications. (2022). Bt Eggplant. ISAAA.org. https://www.isaaa.org/resources/publications/pocketk/48/default.asp

Jabborova, D., Kannepalli, A., Davranov, K., Narimanov, A. A., Enakiev, Y. I., Syed, A., Elgorban, A. M., Bahkali, A. H., Wirth, S., Sayyed, R. Z., & Gafur, A. (2021). Co-inoculation of rhizobacteria promotes growth, yield, and nutrient contents in soybean and improves soil enzymes and nutrients under drought conditions. Scientific Reports, 11(1). https://doi.org/10.1038/s41598-021-01337-9

Kuila, D., & Ghosh, S. (2022). Aspects, problems and utilization of Arbuscular Mycorrhizal (AM) application as bio-fertilizer in sustainable agriculture. Current Research in Microbial Sciences, 3, 100107. https://doi.org/10.1016/j.crmicr.2022.100107

Lohman, M., Ziegler-Ulsh, C., & Douds, D. (2018, November 12). How to Inoculate Arbuscular Mycorrhizal Fungi on the Farm, Part 1. Rodale Institute. https://rodaleinstitute.org/science/articles/how-to-innoculate-arbuscular-mycorrhizal-fungi-on-the-farm-part-1/

Philippine Statistics Authority. (2023). 2017-2021 Crops Statistics of the Philippines (ISSN-2021-0487). https://psa.gov.ph/sites/default/files/Crops%20Statistics%20of%20the%20Philippines%2C%202017-2021.pdf

Phillips, J. L., & Hayman, D. S. (1970). Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 55(1), 158-IN18. https://doi.org/10.1016/s0007-1536(70)80110-3

Sharma, M., Saini, I., Kaushik, P., Aldawsari, M. S., Balawi, T. A., & Alam, P. (2021). Mycorrhizal fungi and Pseudomonas fluorescens application reduces root-knot nematode (Meloidogyne javanica) infestation in eggplant. Saudi Journal of Biological Sciences, 28(7), 3685–3691. https://doi.org/10.1016/j.sjbs.2021.05.054

Suralta, R. R., Inukai, Y., & Yamauchi, A. (2010). Dry matter production in relation to root plastic development, oxygen transport, and water uptake of rice under transient soil moisture stresses. Plant and Soil, 332(1–2), 87–104. https://doi.org/10.1007/s11104-009-0275-8

Tagliavini, M., Veto, L., & Looney, N. (1993). Measuring root surface area and mean root diameter of peach seedlings by digital image analysis. HortScience, 28(11), 1129–1130. https://doi.org/10.21273/hortsci.28.11.1129

Tomazelli, D. (2022, April 20). Inoculation of Arbuscular Mycorrhizal Fungi Improves Growth and Photosynthesis of Ilex paraguariensis (St. Hil) Seedlings. https://www.scielo.br/j/babt/a/PGVqknMdCLbD5cTSN9QVwtC/?lang=en

Wei, M., Zhang, M., Huang, G., Yuan, Y., Fu, C., & Yu, L. (2020). Coculture with two Bacillus velezensis strains enhances the growth of Anoectochilus plants via promoting nutrient assimilation and regulating rhizosphere microbial community. Industrial Crops and Products, 154, 112697. https://doi.org/10.1016/j.indcrop.2020.112697

Wu, J., & Guo, Y. (2014). An integrated method for quantifying root architecture of field-grown maize. Annals of Botany, 114(4), 841–851. https://doi.org/10.1093/aob/mcu009

Wu, Q., Srivastava, A. K., & Zou, Y. (2013). AMF-induced tolerance to drought stress in citrus: A review. Scientia Horticulturae, 164, 77–87. https://doi.org/10.1016/j.scienta.2013.09.010

Yao, Q., Wang, L., Zhu, H., & Chen, J. C. (2009). Effect of arbuscular mycorrhizal fungal inoculation on root system architecture of trifoliate orange (Poncirus trifoliata L. Raf.) seedlings. Scientia Horticulturae, 121(4), 458–461. https://doi.org/10.1016/j.scienta.2009.03.013