Sterilization of Zeolite Media to Increase Arbuscular Mycorrhizal Fungi Colonization in Corn and Sorghum Plants

Nur  Robifahmi; Muftia  Hanani; Taufiq  Bachtiar; Anggi Nico  Flatian; Ania  Citraresmini

doi:10.58222/jtm.v3i1.71

Authors

Nur Robifahmi Pusat Riset Teknologi Proses Radiasi. Badan Riset Inovasi Nasional. Jl. Lebak Bulus Raya No. 49, Jakarta Selatan 12440, Indonesia
Muftia Hanani Pusat Riset Teknologi Proses Radiasi. Badan Riset Inovasi Nasional. Jl. Lebak Bulus Raya No. 49, Jakarta Selatan 12440, Indonesia
Taufiq Bachtiar Pusat Riset Lingkungan dan Teknologi Bersih. Badan Riset Inovasi Nasional. Kawasan Puspitek Gd. 820, Serpong 15314, Tangerang Selatan
Anggi Nico Flatian Pusat Riset Lingkungan dan Teknologi Bersih. Badan Riset Inovasi Nasional. Kawasan Puspitek Gd. 820, Serpong 15314, Tangerang Selatan
Ania Citraresmini Pusat Riset Lingkungan dan Teknologi Bersih. Badan Riset Inovasi Nasional. Kawasan Puspitek Gd. 820, Serpong 15314, Tangerang Selatan

DOI:

https://doi.org/10.58222/jtm.v3i1.71

Keywords:

Gamma Radiation, Mycorrhiza Fungi, Colonisation, Growing Media

Abstract

Productivity of crops such as maize and sorghum is often constrained by low soil fertility, especially in terms of nutrient availability. Arbuscular Mycorrhiza Fungi (FMA) can improve nutrient absorption and support plant growth through a symbiotic relationship with plant roots. However, the success of FMA colonisation is strongly influenced by the quality of the growing medium, which is often contaminated with competing microorganisms that inhibit its effectiveness. To address this issue, this study aimed to evaluate the effect of sterilisation methods on the colonisation of FMA in maize and sorghum plants. The sterilisation methods tested were autoclaving and gamma radiation. This study used a completely randomised design with 8 treatment combinations and 5 replications. Zeolite media was sterilised using autoclaving and gamma radiation at a dose of 50 kGy. Results showed that sterilisation with gamma radiation produced a higher number of mycorrhiza spores and increased root colonisation in maize and sorghum compared to autoclaving. Gamma radiation applied to one sorghum seedling resulted in the highest spore count, while two seedlings in one pot reduced colonisation efficiency due to competition for nutrients. Gamma radiation was more effective in improving the quality of growing media and supporting mycorrhiza colonisation, ultimately enhancing overall plant growth and nutrient absorption.

References

Budi, Sri Wilarso. 2012. “Pengaruh Sterilisasi Media Dan Dosis Inokulum Terhadap Pembentukan Ektomikoriza Dan Pertumbuhan Shorea Selanica Blume.” Jurnal Silvikultur Tropika 03 (02): 76–80.

Camille S. Delavaux, Lauren M. Smith-Ramesh, Sara E. Kuebbing. 2017. “Beyond Nutrients: A Meta-Analysis of the Diverse Effects of Arbuscular Mycorrhizal Fungi on Plants and Soils.” Ecology 98 (8): 2111–19. https://doi.org/10.1111/ijlh.12426.

Campo, Sonia, Héctor Martín-cardoso, Marta Olivé, Eva Pla, Mar Catala-forner, Maite Martínez-eixarch, and Blanca San Segundo. 2020. “Effect of Root Colonization by Arbuscular Mycorrhizal Fungi on Growth , Productivity and Blast Resistance in Rice.” Rice 13 (42): 1–14.

Diagne, Nathalie, Mariama Ngom, Pape Ibrahima Djighaly, and Dioumacor Fall. n.d. “Roles of Arbuscular Mycorrhizal Fungi on Plant Growth and Performance : Importance in Biotic And.”

F.J. Ley, J. Bleby, Coates, Marie E, J.S. Paterson. 1969. “Sterilization Of Laboratory Animal Diets Using Gamma Radiation.” Lab Anim 3: 221–54.

Hasanain, Fatima, Katharina Guenther, Wayne M Mullett, Fatima Hasanain, Katharina Guenther, Wayne M Mullett, and Emily Craven. 2014. “Gamma Sterilization of Pharmaceuticals −− A Review of the Irradiation of Excipients , Active Pharmaceutical Ingredients , and Final Drug Product Formulations Gamma Sterilization of Pharmaceuticals — A Review of the Irradiation of Excipients , Active Pharmaceutical Ingredients ,.” https://doi.org/10.5731/pdajpst.2014.00955.

Hassan Makarian, Vahid Poozesh, Hamid Reza Asghari & Meisam Nazari. 2016. “Interaction Effects of Arbuscular Mycorrhiza Fungi and Soil Applied Herbicides on Plant Growth.” Communications in Soil Science and Plant Analysis 3624 (February): 1–30. https://doi.org/10.1080/00103624.2016.1146744.

Hassena, Ameni Ben, Mohamed Zouari, Wahid Khabou, and Nacim Zouari. 2022. “Impact of Inoculation with Single and Mixed Species of Arbuscular Mycorrhizal Fungi on the Soil Fertility and the Nutrient Uptake of Young Olive Plants,” no. September: 48–54.

Kalamulla, Ruwanthika, Samantha C Karunarathna, Saowaluck Tibpromma, Mahesh C A Galappaththi, Nakarin Suwannarach, Steven L Stephenson, Suhail Asad, Ziad Salman Salem, and Neelamanie Yapa. 2022. “Arbuscular Mycorrhizal Fungi in Sustainable Agriculture.” Sustainability, 1–14.

Mansur, Irdika, Abimanyu Dipo Nusantara, Rr. Yudhy Harini Bertham. 2012. Bekerja Dengan Fungi Mikoriza. Edited by Nia Januarini. Cetakan Pe. Bogor, Indonesia: SEAMEO BIOTROP (.

Mcnamara, N P, H I J Black, N A Beresford, and N R Parekh. 2003. “Effects of Acute Gamma Irradiation on Chemical , Physical and Biological Properties of Soils.” Applied Soil Ecology 24: 117–32. https://doi.org/10.1016/S0929-1393(03)00073-8.

Nurrobifahmi, Anas, Iswandi, and Yadi Setiadi. 2017. “Pengaruh Metode Sterilisasi Radiasi Sinar Gamma Co-60 Dan Autoklaf Terhadap Bahan Pembawa , Viabilitas Spora Gigaspora Margarita Dan Ketersediaan Fe , Mn , Dan Zn Effect of Sterilization Using Gamma Rays and Autoclave on Carrier Materials , Viability of S.” Jurnal Tanah Dan Iklim 41 (1): 1–8.

Saia, Sergio, Paolo Ruisi, Veronica Fileccia, Giuseppe Di Miceli, Gaetano Amato, and Federico Martinelli. 2015. “Metabolomics Suggests That Soil Inoculation with Arbuscular Mycorrhizal Fungi Decreased Free Amino Acid Content in Roots of Durum Wheat Grown under N-Limited , P-Rich Field Conditions,” 1–15. https://doi.org/10.1371/journal.pone.0129591.

Sally E. Smith, David J. Read. 2008. Mycorrhizal Symbiosis (3rd Ed.). (3rd Editi. London: Academic Press (Elsevier).

Sparks, Beverly J. 1984. “Product Sterilization.” AORN JOURNAL 40 (3): 388–90.

Zhang, Shixiu, Shuyan Cui, Xiaoming Gong, Liang Chang, Shuxia Jia, Xueming Yang, Donghui Wu, and Xiaoping Zhang. 2016. “Effects of Gamma Irradiation on Soil Biological Communities and C and N Pools in a Clay Loam Soil.” Applied Soil Ecology 108: 352–60. https://doi.org/10.1016/j.apsoil.2016.09.007.