ANTAGONISTIC ABILITY OF ENTOMOPATHOGENIC FUNGI METARHIZIUM ANISOPLIAE AND BEAUVERIA BASSIANA AGAINST MORTALITY OF WOOD GRASSHOPPER (VALANGA NIGRICORNIS)

Authors

  • Chairunnisah J Lamangantjo Universitas Negeri Gorontalo, Indonesia
  • Winangsi Din Uno Universitas Negeri Gorontalo, Indonesia
  • Intan Zulfatadillah Mursalin Universitas Negeri Gorontalo, Indonesia
  • Maryam Pakaya Universitas Negeri Gorontalo, Indonesia

DOI:

https://doi.org/10.58222/jtm.v4i2.87

Keywords:

Antagonistic, Beauveria bassiana, Entomopathogenic, Metarhizium anisopliae

Abstract

The entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana are potential biological control agents for suppressing the population of the wood grasshopper (Valanga nigricornis). This study aims to evaluate the antagonistic ability of the fungi M. anisopliae and B. bassiana against the wood grasshopper (Valanga nigricornis). This study is a descriptive study with a quantitative approach, applying inoculum suspension at four concentration levels (P1, P2, P3, and P4) and observing insect mortality and colony growth in culture media. The results showed that the P4 suspension treatment produced the highest mortality of 92% with a probit value of 1.28, while mortality in other treatments ranged from 0 to 75%. The highest infection viability showed that in the P4 treatment, the CFU value was 3.0×106 for M. anisopliae and 1.4×106 for B. bassiana. These findings indicate that high inoculum concentrations increase the antagonistic effectiveness and infection ability of entomopathogenic fungi.

References

Bagariang, W., Kurniati, A., Lestrari, T. M. P., Mahmudah, D., Suyanto, H., & Cahyana, N. A. (2023). Uji Media Padat Beauveria Bassiana Terhadap Mortalitas, Pembentukan Pupa Dan Kemunculan Imago Spodoptera litura Fabr. Agro Wiralodra, 6(1), 1–8. https://doi.org/10.31943/agrowiralodra.v6i1.86

Bouri, M., Arslan, K. S., & Şahin, F. (2023). Climate-Smart Pest Management in Sustainable Agriculture: Promises and Challenges. Sustainability, 15(5), 4592. https://doi.org/10.3390/su15054592

Chatterjee, S. (2022). How Hard Did That Sting? Estimating the Economic Costs of Locust Attacks on Agricultural Production †. Applied Economic Perspectives and Policy, 44(1), 434–459. https://doi.org/10.1002/aepp.13127

Dakhel, W. H., Latchininsky, A. V., & Jaronski, S. T. (2019). Efficacy of Two Entomopathogenic Fungi, Metarhizium brunneum, Strain F52 Alone and Combined with Paranosema locustae against the Migratory Grasshopper, Melanoplus sanguinipes, under Laboratory and Greenhouse Conditions. Insects, 10(4), 94. https://doi.org/10.3390/insects10040094

de Paula, A. R., Silva, L. E. I., Ribeiro, A., da Silva, G. A., Silva, C. P., Butt, T. M., & Samuels, R. I. (2021). Metarhizium anisopliae blastospores are highly virulent to adult Aedes aegypti, an important arbovirus vector. Parasites & Vectors, 14(1), 555. https://doi.org/10.1186/s13071-021-05055-z

Devlet, A. (2021). Modern agriculture and challenges. Frontiers in Life Sciences and Related Technologies, 2(1), 21–29. https://doi.org/10.51753/flsrt.856349

Fanani, M. Z., Judijanto, L., Tobing, O. L., Riono, Y., Sari, L. A., Juhandi, D., & Lada, Y. G. (2025). Pertanian Berkelanjutan. PT. Sonpedia Publishing Indonesia.

Fronza, E., Specht, A., Heinzen, H., & de Barros, N. M. (2017). Metarhizium (Nomuraea) rileyi as biological control agent. Biocontrol Science and Technology, 27(11), 1243–1264. https://doi.org/10.1080/09583157.2017.1391175

Gebremariam, A., Chekol, Y., & Assefa, F. (2022). Extracellular enzyme activity of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae and their pathogenicity potential as a bio-control agent against whitefly pests, Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera: Aleyrodidae). BMC Research Notes, 15(1), 117. https://doi.org/10.1186/s13104-022-06004-4

Grijalba, E. P., Espinel, C., Cuartas, P. E., Chaparro, M. L., & Villamizar, L. F. (2018). Metarhizium rileyi biopesticide to control Spodoptera frugiperda: Stability and insecticidal activity under glasshouse conditions. Fungal Biology, 122(11), 1069–1076. https://doi.org/10.1016/j.funbio.2018.08.010

Mani, M. (2022). Pest Management in Horticultural Crops Under Protected Cultivation. In Trends in Horticultural Entomology (pp. 387–417). Springer Nature Singapore. https://doi.org/10.1007/978-981-19-0343-4_12

Mascarin, G. M., & Jaronski, S. T. (2016). The production and uses of Beauveria bassiana as a microbial insecticide. World Journal of Microbiology and Biotechnology, 32(11), 177. https://doi.org/10.1007/s11274-016-2131-3

Mulvihill, S. (2021). The Vegetable Garden Pest Handbook: Identify and Solve Common Pest Problems on Edible Plants-All Natural Solutions! Cool Springs Press.

Paschapur, A. U., Samal, I., Ganiger, P. C., Guru, P. N., & Singh, A. K. (2025). Insect Pests of Millet Crops and Their Integrated Management. In Minor Millets (pp. 161–181). Springer Nature Singapore. https://doi.org/10.1007/978-981-96-4265-6_7

Pertiwi, S. A., & Nanang Tri Haryadi. (2022). Uji Toksisitas Jamur Metarhizium anisopliae terhadap Hama Ulat Krop Kubis Crocidolomia binotalis Zell. JURNAL AGRI-TEK : Jurnal Penelitian Ilmu-Ilmu Eksakta, 23(2), 15–20. https://doi.org/10.33319/agtek.v23i2.116

Prastyaningsih, S. R., & Juliarti, A. (2023). Jenis-Jenis Hama Serangga Gaharu (Aquilaria mallacensis Lamk) di Desa Kuapan, Kecamatan Tambang, Kabupaten Kampar, Provinsi RIAU. Jurnal Karya Ilmiah Multidisiplin (JURKIM), 3(1), 9–15. https://doi.org/10.31849/jurkim.v3i1.12043

Ramdani, C., El Fakhouri, K., Boulamtat, R., Bouharroud, R., Mesfioui, A., Al-Jaboobi, M., & El Bouhssini, M. (2022). Entomopathogenic fungi as biological control agents of Dactylopius opuntiae (Hemiptera: Dactylopiidae) under laboratory and greenhouse conditions. Frontiers in Sustainable Food Systems, 6. https://doi.org/10.3389/fsufs.2022.997254

Susandi, Y., Salakia, C. L., & Watung, J. F. (2023). Aplikasi Metarhizium anisopliae Dan Azadirachta indica A. Juss Untuk Mengendalikan Nephotettix virescens D. Sebagai Serangga Vektor Penyakit Tungro Pada Tanaman Padi. Jurnal MIPA, 12(2), 68–73.

Teweloipaky, W. Y., Wattimena, C. M. A., & Latumahina, F. S. (2024). Diagnosa Jenis Hama Yang Merusak Tanaman Titi (Gmelina moluccana Blume) Pada Demplot Sumber Benih Desa Hatusua. HUTAN TROPIKA, 19(1), 164–173. https://doi.org/10.36873/jht.v19i1.14030

Tobing, S. S. L., Marheni, & Hasanuddin. (2015). Effectivity test Metarhizium anisopliae Metch. and Beauveria bassiana Bals. against Oriental leafworm moth (Spodoptera litura F.) in Soybean (Glicyne max L.) at Screen House. Agroekoteknologi, 4(1), 1659–1665.

Yasin, M., Khan, A., Qayyum, M. A., Yousuf, H. M. B., Mehfooz, A., & Hunter, D. (2024). Biological control of locusts and grasshoppers: A review. Journal of Orthoptera Research, 33(2), 289–304. https://doi.org/10.3897/jor.33.114472

Yin, F., Xiao, M., Berestetskiy, A., & Hu, Q. (2021). The Metarhizium anisopliae Toxin, Destruxin A, Interacts with the SEC23A and TEME214 Proteins of Bombyx mori. Journal of Fungi, 7(6), 460. https://doi.org/10.3390/jof7060460

Downloads

Published

2025-10-30

How to Cite

Lamangantjo, C. J., Winangsi Din Uno, Intan Zulfatadillah Mursalin, & Maryam Pakaya. (2025). ANTAGONISTIC ABILITY OF ENTOMOPATHOGENIC FUNGI METARHIZIUM ANISOPLIAE AND BEAUVERIA BASSIANA AGAINST MORTALITY OF WOOD GRASSHOPPER (VALANGA NIGRICORNIS). Journal of Tropical Mycorrhiza, 4(2), 45–50. https://doi.org/10.58222/jtm.v4i2.87

Issue

Section

Articles