Single-Spore Culture of Racocetra crispa Using a Legume Tree Species

Faisal Danu Tuheteru; Deli; Husna Husna; Asrianti Arif; Wiwin Rahmawati Nurdin

doi:10.58222/jtm.v5i1.118

Authors

Faisal Danu Tuheteru Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.
Deli Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.
Husna Husna Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.
Asrianti Arif Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.
Wiwin Rahmawati Nurdin Forestry Department, Faculty of Forestry and Environmental Sciences, Universitas Halu Oleo, Kendari, Indonesia.

DOI:

https://doi.org/10.58222/jtm.v5i1.118

Keywords:

Glomeromycotina, Albizia saponaria, AMF propagation

Abstract

The propagation of Racocetra crispa using tree-like legume host plants has not yet been widely undertaken. This study aims to investigate the sporulation of R. crispa in single-spore cultures using legume tree host plants, namely sengon (Paraserianthes falcataria), albizia (Albizia saponaria), johar (Cassia siamea), cajanus (Cajanus cajan), and lamtoro (Leucaena leucocephala). The study was conducted at the Greenhouse of the Indonesian Mycorrhiza Association (AMI) Southeast Sulawesi Branch and the Forestry Department Laboratory of Halu Oleo University from November 2025 to February 2026. The study employed a completely randomised design (CRD) with 5 treatments and 3 replicates. The variables observed included culture success, AMF colonisation of the roots, and the number of spores formed at 3 months, 5 months, and after drying 10 g of medium. The results of the study showed that the A. saponaria host plant treatment yielded the best results, with a culture success rate of 86.67%, the highest AMF colonisation of 35.83%, and the highest number of spores at 5 months and after drying 10g of medium. Conversely, L. leucocephala showed the lowest results, with a culture success rate and spore count of 0%. The best host plant for the propagation of Racocetra crispa is A.saponaria.

References

Aye, P. A. dan Adegun, M. K. 2013. Chemical composition and some functional properties of moringa, leucaena and gliricidia leaf meals. Agriculture and biology journal of North America. 4(1): 71-77.

Baharuddin, A. 2023. Pengaruh Zat Pengatur Tumbuh Alami Terhadap Perkecambahan Tanaman Lamtoro Teramba [Doctoral dissertation, Universitas Hasanuddin].

Brundrett, M., N. Bougher, B. Dell, Grove, dan Malajczuk. 1996. Working with mycorrhiza in forestry and agriculture. Australian Centre for International Agricultural Research, Canberra.

Delvian, D., dan A. Hartanto. 2021. Improved salt tolerance of lamtoro (Leucaena leucocephala) through the application of indigenous mycorrhiza. International Journal of Forestry Research. 2021(1): 8100480.

Delvian. 2004. Aplikasi cendawan mikoriza arbuskula dalam reklamasi lahan kritis pascatambang. Medan. 5-7.

Himaniarwati, H. 2025. Albizia saponaria: herbal antimikroba alami. Jakarta: Buku lokal literasi bangsa.

Husna, H. 2015. Respon pertumbuhan bibit kayu kuku (Pericopsis mooniana (Thw.) Thw) Terhadap Inokulasi Fungi Mikoriza Arbuskula Lokal. Jurnal Pemuliaan Tanaman Hutan. 9(3): 131-148.

IJdo, M., S. Cranenbrouck, dan S. Declerck. 2011. Methods for large-scale production of AM fungi: Past, present, and future. Mycorrhiza. 21: 1-16.

Mansur I. 2013.Teknik Silvikultur untuk Reklamasi Lahan Bekas Tambang. Bogor (ID): Seameo Biotrop.

Nurmasyitah dan Khairuna. 2017. Aplikasi pupuk NPK dan fungi mikoriza arbuskula (FMA) terhadap P-tersedia tanah, serapan P dan pertumbuhan bibit lada lokal Aceh pada media tanah Inceptisol. Floratek. 12(2):62-74.

Puspitasari, D., K. I. Purwani dan A. Muhibbudin. 2012. Eksplorasi vesicular arbuscular mycorrhiza (VAM) indigenous pada lahan jagung di Desa Torjun, Sampang Madura. Jurnal Sains dan Seni ITS. 1(1): 19-22.

Riyanto, B.A.Y. 2011. Sengon (Paraserianthes falcataria) bermikoriza untuk fitoremediasi timbal (Pb) dalam media tanam tanah tercemar tailing tambang emas rakyat [skripsi]. Universitas Brawijaya. Malang

Sanginga, N., G.D. Bowen, dan S.K.A. Danso.1995. Influence of plant species on mycorrhizal colonization. Biology and Fertility of Soils.

Santos, J.L., R.D. Chaves, dan A.S. Sant’Ana. 2020. Modeling the impact of water activity, pH, and calcium propionate on the germination of single spores of Penicillium paneum. LWT. 133: 110012.

Sari, A. J., dan I. K. Purwani. 2013. Pengaruh mikoriza glomus faciculatum terhadap akumulasi logam timbal (Pb) pada tanaman dahlia pinnata. institut teknologi sepuluh nopember (ITS), Surabaya. Edisi Jurnal Sains dan Seni Pomits. 2 (2):2337-3520.

Selong, J. D., dan Y.H. Lawing. 2023. Evaluasi keberhasilan tanaman revegetasi lahan pasca tambang CV. Indra Berjaya Kecamatan Anggana Kabupaten Kutai Kartanegara Provinsi Kalimantan Timur. Jurnal Geologi Pertambangan (JGP). 29(1): 1-12.

Smith, S. E and D. J. Read, 2008. Mycorrhizal symbiosis third edition. Academic Press (AP). New York.

Souza FA da, Silva IR, de Barros-Barreto MBB, dan F. Oehl. 2018. Racocetra crispa (Glomeromycotina) delimited by integrative evidence based on morphology, long continuous nuclear rDNA sequencing and phylogeny. Mycological Progress. 17: 999-1011.

Tuheteru, F. D., Husna, dan Arif.A. 2011. Respon pertumbuhan dan ketergantungan albizia (Albizia saponaria) (lour.) Miq terhadap inokulasi fungi mikoriza arbuskula lokal sulawesi tenggara pada media tanah pasca tambang nikel. Berita Biologi. 10(5): 605-611.

Tuheteru, F. D., W. R. Nurdin, A. Himawan, E.J. Tuheteru, S. Mulyono dan A. Arif. 2025. Native arbuscular mycorrhizal fungi promote the growth of Vitex cofassus seedlings in post-asphalt mining soil media. Journal of Degraded and Mining Lands Management. 12(2): 7225-7233.

Wicaksono, M. I., M. Rahayu dan Samanhudi. 2014. Pengaruh pemberian mikoriza dan pupuk organik terhadap perumbuhan bawang putih. Ilmu-Ilmu Pertanian. 29(1):35-44.