Production of Arbuscular Mycorrhizal Fungi Spores (AMF) Glomus Coronatum and Glomus Claroideum With Ab Mix Nutrition

Yevita Rombe; Husna Husna; Faisal Danu Tuheteru; Asrianti  Arif

doi:10.58222/jtm.v1i2.33

Authors

Yevita Rombe Department of Forestry, Faculty of Forestry and Environmental Sciences, Halu Oleo University, Kendari, Southeast Sulawesi. 93121, Indonesia.
Husna Husna Department of Forestry, Faculty of Forestry and Environmental Sciences, Halu Oleo University, Kendari, Southeast Sulawesi. 93121, Indonesia.
Faisal Danu Tuheteru jurusan kehutanan universitas halu oleo
Asrianti Arif Department of Forestry, Faculty of Forestry and Environmental Sciences, Halu Oleo University, Kendari, Southeast Sulawesi. 93121, Indonesia.

DOI:

https://doi.org/10.58222/jtm.v1i2.33

Keywords:

Glomus claroideum, AB nutrition mix,Spore production.

Abstract

Arbuscular Mycorrhizal Fungi (AMF) is an obligate fungus that has a symbiosis with plant roots and can stimulate plant growth and productivity so that AMF has the potential to be developed as a biological fertilizer. AMF propagation is influenced by many factors including fertilization. This study aims to determine the effect of giving AB mix nutritional doses on the production of AMF types of Glomus coronatum and Glomus claroideum. The research was conducted at the Plastic House of the Indonesian Mycorrhizal Association Southeast Sulawesi Branch and the Laboratory of the Department of Forestry, Faculty of Forestry and Environmental Sciences, UHO in September - December 2022 using a factorial Completely Randomized Design (CRD) with two factors, namely the first factor was AMF type including Glomus coronatum and Glomus claroideum and the second factor is AB mix nutrition including control, 2.5 ml/1000 ml water, 5 ml/1000 ml water. The results showed that the interaction treatment of AMF Glomus claroideum and the provision of 2.5 ml AB mix nutrition increased the number of AMF spores by 35 spores/5 g zeolite and root colonization by 69.16%.

References

Alotaibi, M.O., A.M. Saleh., R.L. Sobrinho., M.S. Sheteiwy., A.M. El-sawah., A.E. Mohammed and H.A. Elgawad. 2021. Arbuscular mycorrhizae mitigate aluminum toxicity and regulate proline metabolism in plants grown in acidic soil. Journal of Fungi. 7(531):1-15.

Asmarahman, C., S.W. Budi., I. Wahyudi dan E. Santoso. 2018. Identifikasi mikroba potensial fungi mikoriza Indonesia tbk . Cibinong , Bogor , Jawa barat . Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan. 8(3):79–85.

Brundrett, M., N. Bougher., B. Dell., T. Grove., And N. Malajczuk . 1996. Working with mycorrhizas in forestry and agriculture. Aclar Monograph. 32(1): 374.

Delvian. 2008. Pengaruh spesies inang dan sumber nutrisi terhadap produksi spora fungi mikoriza arbuskula. Jurnal Nature Indonesia. 10 (2): 70-72.

Dodd, J. C., C. L. Boddington, A. Rodriguez, C. GonzalezChavez, and I. Mansur. 2000. Mycelium of arbuscularmycorrhizal fungi (AMF) from diﬀ erent genera: form,function and detection. Plant Soil. (226): 131–151.

Ghosh, A., and S. Dutta. 2018. Beneficial aspects of arbuscular mycorrhizal fungus rhizophagus irregularis on plant growth and vigour of arachis hypogaea l. International Journal of Pharmacy and Biological Sciences. 8(4): 605-611.

Ginanjar, A., L.S. Banu dan S. Suryani. 2021. Respon sawi samhong (Brassica rapa subsp chinensis) terhadap urin kelinci dan pupuk organik cair kulit nanas dalam ab mix pada sistem wick. Jurnal Ilmiah Respati. 12(2): 147-162.

Hasanah, U.S., Husna, dan F.D. Tuheteru. 2016. Sporulasi Fungi Mikoriza Arbuskula lokal Asal Rizosfer Kayu Kuku [Pericopsis mooniana (Thw)Thw.] dengan Pemberian Takaran Terabuster yang Berbeda [skripsi]. Universitas Haluoleo. Kendari.

Hidayat, C. 2013. Studi Biodiversitas Fungi Mikoriza Arbuskula pada Tumbuhan Bawah di Tegakan Sengon (Falcataria moluccana (Miq.) Barneby & Grimes) [skripsi]. Institut Pertanian Bogor. Bogor.

Husna, 2015. Potensi Fungi Mikoriza Arbuskula (FMA) Lokal dalam Konservasi Ex-Situ Jenis Terancam Punah Kayu Kuku [Pericopsis mooniana (Thw) Thw.].[disertasi]. Sekolah Pascasarjana Institut Pertanian Bogor. Bogor.

Husna, Mey D, dan Yulistiati, T. 2004. Pengaruh inang dan media tanam terhadap perbanyakan spora CMA asal Muna dan Kendari. Agriplus. 14(3): 193-198.

Husna., F. D. Tuheteru and A. Arif. 2018. Arbuscular mycorrhizal fungi symbiosis and conservation of endangered tropical legume trees. In Root Biology. 52: 465-486.

Husna., F. D. Tuheteru., A. Arif dan A. F. Renggaala. 2017. Sporulasi fungi mikoriza arbuskula lokal asal rizosfer kayu kuku [Pericopsis mooniana (thw.) Thw] yang dipengaruhi takaran hyponex merah. Ecogreen. 3(2): 79 – 87.

Husna., F. D. Tuheteru., A. Arif., Basrudin and Albasri. 2021. Biodiversity of arbuscular mycorrhizal fungi in tropical Indonesia. In: An Introduction to Microorganisms. 201-247.

Husna., F.D. Tuheteru and A. Arif. 2021. Arbuscular mycorrhizal fungi to enhance the growth of tropical endangered species Pterocarpus indicus and Pericopsis mooniana in post gold mine field in southeast Sulawesi, indonesia. Biodiversitas Journal of Biological Diversity. 22(9): 3844-3853.

Husna., F.D. Tuheteru and E. Wigati. 2017. Growth response and dependency of endangered nedun tree species (Pericopsis mooniana) affected by indigenous arbuscular mycorrhizal fungi inoculation. Nusantara Bioscience. 9(1): 57-61.

Husna., F.D. Tuheteru., A. Arif And Solomon. 2019. Improvement of early growth of endemic Sulawesi trees species Kalappia celebica by arbuscular mycorrhizal fungi in gold mining tailings. In Iop Conference Series: Earth And Environmental Science. 394(1): 1-5.

Husna., R.S.W. Budi., I. Mansur and C. Kusmana. 2016. Growth and nutrient status of kayu kuku [Pericopsis mooniana (thw.) Thw] with mycorrhiza in soil media of nickel post mining site. Pakistan Journal of Biological Sciences: Pjbs. 19(4): 158-170.

Husna., S. Wilarso, I. Mansur dan C. Kusmana. 2015. Respon pertumbuhan bibit kayu kuku (Pericopsis mooniana (Thw.) Thw) terhadap inokulasi fungi mikoriza arbuskula lokal. Jurnal Pemuliaan Tanaman Hutan. 9(3):131-148.

Indriani, S., H.A. Ekamawanti dan Burhanuddin. 2019. Produksi inokulum mikoriza arbuskula dari kedalaman gambut berbeda yang diberi cuka organik dengan inang jagung (Zea mays L.). Jurnal Hutan Lestari. 7(2): 822 – 834.

Kobayashi, Y., T. Maeda., K. Yamaguchi., H. Kameoka., S. Tanaka dan T. Ezawa. 2018. The genome of rhizophagus clarus hr1 reveals a common genetic basis for auxotrophy among arbuscular mycorrhizal fungi. Bmc Genomics. 19( 456): 1–11.

Mattjik, A dan Sumertajaya. 2013. Perancangan percobaan dengan aplikasi SAS dan Minitab. Institut Pertanian Bogor.

Muis, R., M. Ghulamahdi., M. Melati., P. Purwono dan I. Mansur. 2016. Kompatibilitas fungi mikoriza arbuskular dengan tanaman kedelai pada budi daya jenuh air. Jurnal Penelitian Pertanian Tanaman Pangan. 35(3): 125 - 157.

Nusantara, A.D., C. Kusmana., I. Mansur., L.K. Darusman dan S. Soedarmadi. 2010. Pemanfaatan bahan bio-anorganik untuk memproduksi biomassa hijauan pakan dan inokulan fungi mikoriza arbuskula. Media Peternakan. 33(3): 162-162.

Pacioni, G, 1992. Wet-sieving and decanting techniques for the extraction of spor es of vesicular-arbuscular fungi. In: Methods in Microbiology. 24(16): 317-322.

Putri, K.P dan Nurhasybi. 2010. Pengaruh jenis media organik terhadap kualitas bibit takir (Duabanga moluccana). Jurnal Penelitian Hutan Tanaman. 7(3):141-146.

Rengganis, D. 2013. Studi Keanekaragaman Genus Fungi Mikoriza Arbuskula di Sekitar Perakaran Pohon Jabon (Anthocephalus cadamba Robx Miq.) Alami [skripsi]. IPB. Bogor.

Rini, M.V., S. Wahyudi dan Sugiatno. 2020. Pengaruh jumlah tanaman inang terhadap infeksi akar d an produksi spora fungi mikoriza arbuskula. Jurnal Agrotropika. 19(2): 70- 75.

Sari, S dan W. Indrawati. 2019. Aplikasi berbagai jenis pupuk organik terhadap karakter FMA pada rhizosfer tebu bud chip. Jurnal Penelitian Pertanian Terapan. 17 (3): 1-10.

Sari, S.W., S. Safruddin dan D.W. Purba. 2019. Pengaruh pemberian ekstrak daun kelor dan nutrisi ab-mix terhadap pertumbuhan dan produksi tanaman seledri (Apium graveolens L.) Secara hidroponik dengan sistem wick. Jurnal Penelitian Pertanian. 15(3): 22-31.

Sartini. 2004. Mikoriza arbuskula dan kascing Pengaruh terhadap Pertumbuhan tanaman. Jurnal Bidang Ilmu Pertanian. 36–38.

Selvakumar, G., C.C. Shagol, Y. Kang., B.N. Chung, S.G. Han and T.M. Sa. 2018. Arbuscular mycorrhizal fungi spore propagation using single spore as starter inoculum and a plant host. Journal of Applied Microbiology. 124: 1556 – 1565.

Setiawan, N.D. 2018. Otomasi pencampur nutrisi hidroponik sistem ntf (nutrient film technique) berbasis arduino mega 2560. Jurnal Teknik Informatika Unika Santo Thomas. 3(2): 78-82.

Sieverding, E. 1991. Vesicular-Arbuscular Mycorrhizae Management in Tropical Agrosystem. Germany: Eschborn Germany.

Smith SE, Read DJ. 2008. Mycorrhizal symbiosis. Third ed. New York (US): Academic Press.

Souza, T. 2015. Handbook of arbuscular mychorrhizal fungi springer. New York.

Tuheteru, F. .D., dan Husna. 2011. Pertumbuhan dan biomassa Albizia saponaria yang diinokulasi fungi arbuskula mikoriza lokal Sulawesi Tenggara. Jurnal Silvikultur Tropika. 1(2): 143 – 148.

Tuheteru, F.D. 2003. Aplikasi Asam Humat Terhadap Sporulasi CMA dari Bawah Tegakan Alami Sengon [Paraserianthes falcataria (L.) Nielsen] Asal Maluku [skripsi]. Institut Pertanian Bogor. Bogor.

Uswatun, S.H, 2017. Sporulasi Lokal Asal Rizosfer Kayu Kuku [Pericopsis mooniana (Thw. Thw.] Dengan Pemberian Takaran Terabuster yang Berbeda [skripsi]. Universitas Halu Oleo. Kendari.

Wang, J., G.G. Wang., B. Zhang., Z. Yuan., Z. Fu., Y. Yuan., L. Zhu., S. Ma and J. Zhang. 2019. Arbuscular mycorrhizal fungi associated with tree species in a planted forest of eastern china. Mdpi. 10: 1–14.

Yuwati., T. Wira dan W. Imaningsih. 2021. Peningkatan pertumbuhan semai sengon menggunakan fungi mikoriza arbuskula asli gambut tropis. Jurnal Galam. 1(2): 93-107.